製品の概要

  • 製品名
    Anti-Calreticulin antibody
    Calreticulin 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to Calreticulin
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, WB, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Hamster, Dog, Human
    交差が予測される動物種: Rabbit, Cow, Pig, Zebrafish, Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Mouse Calreticulin aa 24-43.
    Sequence:

    KEQFLDGDAWTNRWVESKHK


    (Peptide available as ab5838)

  • ポジティブ・コントロール
    • AtT-20 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1mg/ml BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgY
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab94935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. ab37382-Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 60-66 kDa (predicted molecular weight: 48 kDa).Can be blocked with Calreticulin peptide (ab5838).
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • 配列類似性
    Belongs to the calreticulin family.
  • ドメイン
    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • 細胞内局在
    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

画像

  • All lanes : Anti-Calreticulin antibody (ab94935) at 1/2000 dilution

    Lane 1 : A-549
    Lane 2 : HEK 293
    Lane 3 : Mouse Brain

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml

    Predicted band size : 48 kDa
  • Anti-Calreticulin antibody (ab94935) at 1/1000 dilution + AtT-20 cell lysate

    Predicted band size : 48 kDa

参考文献

ab94935 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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