Anti-Calreticulin 抗体 (ab94935)
Key features and details
- Chicken polyclonal to Calreticulin
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgY
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Calreticulin antibody
Calreticulin 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to Calreticulin -
由来種
Chicken -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Cow, Pig, Zebrafish, Cynomolgus monkey -
免疫原
Synthetic peptide corresponding to Mouse Calreticulin aa 24-43.
Sequence:KEQFLDGDAWTNRWVESKHK
(Peptide available asab5838) -
ポジティブ・コントロール
- WB: A-549, HEK-293 cell lysate; Mouse Brain lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94935の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 60-66 kDa (predicted molecular weight: 48 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 60-66 kDa (predicted molecular weight: 48 kDa). |
ターゲット情報
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機能
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. -
配列類似性
Belongs to the calreticulin family. -
ドメイン
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
細胞内局在
Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. - Information by UniProt
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参照データベース
- Entrez Gene: 281036 Cow
- Entrez Gene: 102121336 Cynomolgus monkey
- Entrez Gene: 811 Human
- Entrez Gene: 12317 Mouse
- Entrez Gene: 100381266 Pig
- Entrez Gene: 100009050 Rabbit
- Entrez Gene: 30248 Zebrafish
- Omim: 109091 Human
see all -
別名
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
see all
画像
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All lanes : Anti-Calreticulin antibody (ab94935) at 1/2000 dilution
Lane 1 : A-549 cell lysate
Lane 2 : HEK-293 cell lysate
Lane 3 : Mouse Brain lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml
Predicted band size: 48 kDa
データシートおよび資料
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Datasheet download
参考文献 (1)
ab94935 は 1 報の論文で使用されています。
- Stamm CE et al. Screening Mycobacterium tuberculosis Secreted Proteins Identifies Mpt64 as a Eukaryotic Membrane-Binding Bacterial Effector. mSphere 4:N/A (2019). PubMed: 31167949