The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/400. ABC system. 30mins at RT. Calponin has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast.
Calponin has also been useful in malignant myoepithelium and pleomorphoric adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions.
Use at an assay dependent concentration. PubMed: 20618694
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 20138661
1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC system. 30mins at RT.
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Smooth muscle, and tissues containing significant amounts of smooth muscle.
Belongs to the calponin family. Contains 3 calponin-like repeats. Contains 1 CH (calponin-homology) domain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Calponin antibody [CALP] (ab700)This image is courtesy of an Abreview submitted by Dr Mal Niladri
ab700 at a 1/50 dilution staining rat heart tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed prior to incubation with the antibody overnight at 4°C. An Alex-Fluor ® 488 conjugated goat anti-mouse antibody was used as the secondary. The image was taken with a confocal laser scanning microscope with an additional Differential Interference Contrast (DIC) mode and shows stained Calponin (smooth muscle cells-green) and counterstained nuclei (DAPI-blue) (left panel).DIC (phase) image of the same tissue (right panel) confirm the localization of the calponin staining in the arterial smooth muscle in the heart tissue with very little background.
Immunocytochemistry/ Immunofluorescence - Calponin antibody [CALP] (ab700)This image is a courtesy of Anonymous Abreview
ab700 staining Calponin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in physiological saline solution (PSS) for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An Alexa Fluor®488-conjugated chicken polyclonal to mouse IgG was used as secondary antibody at 1/400 dilution. Image shows combined fluorescent and transmitted light images. Hoechst 33342 was used to stain the cell nuclei (blue).