製品の概要

  • 製品名
    Anti-Calponin antibody [CALP]
    Calponin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CALP] to Calponin
  • アプリケーション
    適用あり: IHC-Fr, Flow Cyt, ICC/IF, IHC-FoFr, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Crude human uterus extract

  • ポジティブ・コントロール
    • Myoepithelium in normal breast glands.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/100 - 1/400. ABC system. 30mins at RT.
Calponin has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin has also been useful in malignant myoepithelium and pleomorphoric adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions.
Flow Cyt Use at an assay dependent concentration. PubMed: 20618694
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20138661
IHC-P 1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC system. 30mins at RT.

ターゲット情報

  • 機能
    Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • 組織特異性
    Smooth muscle, and tissues containing significant amounts of smooth muscle.
  • 配列類似性
    Belongs to the calponin family.
    Contains 3 calponin-like repeats.
    Contains 1 CH (calponin-homology) domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basic calponin antibody
    • Calponin 1 antibody
    • Calponin 1 basic smooth muscle antibody
    • Calponin H1 antibody
    • Calponin H1 smooth muscle antibody
    • Calponin-1 antibody
    • Calponin1 antibody
    • Calponins basic antibody
    • CNN 1 antibody
    • Cnn1 antibody
    • CNN1_HUMAN antibody
    • Epididymis secretory protein Li 14 antibody
    • HEL S 14 antibody
    • Sm Calp antibody
    • SMCC antibody
    • smooth muscle antibody
    see all

画像

  • ab700 at a 1/50 dilution staining rat heart tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue was formaldehyde fixed prior to incubation with the antibody overnight at 4°C. An Alex-Fluor ® 488 conjugated  goat anti-mouse antibody was used as the secondary. The image was taken  with a confocal laser scanning microscope with an additional Differential Interference Contrast (DIC) mode and shows stained Calponin (smooth muscle cells-green) and counterstained nuclei (DAPI-blue) (left panel).DIC (phase) image of the same tissue (right panel) confirm the localization of the calponin staining in the arterial smooth muscle in the heart tissue with very little background.

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  • ab700 staining Calponin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in physiological saline solution (PSS) for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An Alexa Fluor®488-conjugated chicken polyclonal to mouse IgG was used as secondary antibody at 1/400 dilution. Image shows combined fluorescent and transmitted light images. Hoechst 33342 was used to stain the cell nuclei (blue).

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参考文献

This product has been referenced in:
  • Malashicheva A  et al. Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic Aneurysms. Int J Vasc Med 2016:3107879 (2016). ICC . Read more (PubMed: 26904289) »
  • Yuan K  et al. Activation of the Wnt/planar cell polarity pathway is required for pericyte recruitment during pulmonary angiogenesis. Am J Pathol 185:69-84 (2015). Read more (PubMed: 25447046) »

See all 14 Publications for this product

レビューと Q&A

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Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Sample
Human Cell (Human arterial myocytes)
Specification
Human arterial myocytes
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 08 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Smooth muscle cells from mesenteric artery)
Specification
Smooth muscle cells from mesenteric artery
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Nov 17 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart tissue)
Specification
Heart tissue
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Mal Niladri

Verified customer

投稿 Oct 16 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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