The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報IHC-P: 1/200 for 30 mins at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
配列類似性Belongs to the protease inhibitor I27 (calpastatin) family.