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Synthetic peptide corresponding to Human Calpain 5 aa 1-30 (N terminal).
(Peptide available as
ab28280 does not recognize amino-processed Calpain 5, and can be used to discriminate between latent and amino-processed forms. ab28280 will also bind to the non-reduced protein, and thus may have application for immunoprecipitation, immunohistochemistry, and ELISA.
Our Abpromise guarantee covers the use of ab28280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 73, 68 kDa.
1/1000 (colorimetric) - 1/5000 (chemiluminescent). A series of further cleaved forms seen when used against the reduced protein. Dilution optimised using Chromogenic detection.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|