製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Calpain 3
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region within C terminal amino acids 610-640 of Human Calpain 3.

  • ポジティブ・コントロール
    • HL-60 cell lysate; Human skeletal muscle tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Predicted molecular weight: 94 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Calcium-regulated non-lysosomal thiol-protease.
  • 組織特異性
    Isoform I is skeletal muscle specific.
  • 関連疾患
    Defects in CAPN3 are the cause of limb-girdle muscular dystrophy type 2A (LGMD2A) [MIM:253600]. LGMD2A is an autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset.
  • 配列類似性
    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Calcium-activated neutral proteinase 3 antibody
    • calpain 3, (p94) antibody
    • Calpain L3 antibody
    • Calpain large polypeptide L3 antibody
    • Calpain p94 antibody
    • calpain p94, large [catalytic] subunit antibody
    • calpain, large polypeptide L3 antibody
    • Calpain-3 antibody
    • CAN3_HUMAN antibody
    • CANP 3 antibody
    • CANP3 antibody
    • CANPL3 antibody
    • CAPN3 antibody
    • LGMD 2A antibody
    • LGMD2 antibody
    • LGMD2A antibody
    • Lp82 antibody
    • Lp85 antibody
    • MGC10767 antibody
    • MGC11121 antibody
    • MGC14344 antibody
    • MGC4403 antibody
    • Muscle-specific calcium-activated neutral protease 3 antibody
    • muscle-specific calcium-activated neutral protease 3 large subunit antibody
    • nCL-1 antibody
    • New calpain 1 antibody
    • p94 antibody
    see all

画像

  • Anti-Calpain 3 antibody (ab103250) at 1/50 dilution + HL-60 cell lysate at 35 µg

    Predicted band size : 94 kDa
  • ab103250, at 1/50 dilution, staining Calpain 3 in formalin-fixed, paraffin-embedded Human skeletal muscle by Immunohistochemistry using a peroxidase conjugated secondary antibody and DAB staining.

参考文献

This product has been referenced in:
  • Kumchantuek T  et al. Expression and localization of calpain 3 in the submandibular gland of mice. Arch Oral Biol 70:9-15 (2016). WB ; Mouse . Read more (PubMed: 27295390) »

See 1 Publication for this product

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