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Synthetic peptide corresponding to Human Calnexin aa 475-493 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
References regarding specificity
Horner SM et al. Mitochondrial-associated endoplasmic reticulum membranes (MAM) form innate immune synapses and are targeted by hepatitis C virus. Proc Natl Acad Sci U S A 108:14590-5 (2011). PubMed: 21844353
Myhill N et al. The subcellular distribution of calnexin is mediated by PACS-2. Mol Biol Cell 19:2777-88 (2008). PubMed: 18417615
Yoshimura SI et al. Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus. J Cell Sci 114:4105-15 (2001). PubMed: 11739642
Our Abpromise guarantee covers the use of ab66332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|WB||1/500 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).|
|ICC/IF||1/200 - 1/1000.|
ab66332 at 1/1000 dilution staining Calnexin in human Hela cells by immunocytochemistry/ Immunofluorescence. An Alexa Fluor® conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. Green colour in image show positive staining with ab66332.