製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/2000.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/50 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/20 - 1/50.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

Anti-Calmodulin antibody [EP799Y] 画像

  • ab45689 immunoprecipitating Calmodulin. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/10000.

    Lane 1: Human skeletal muscle lysate (10ug)
    Lane 2: Human skeletal muscle lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab45689 in Human skeletal muscle lysate

  • ab45689 staining Calmodulin in NIH/3T3 (mouse embryo) cells by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • ab45689 staining Calmodulin in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Lane 1 : Anti-Calmodulin antibody [EP799Y] (ab45689) at 1/10000 dilution
    Lane 2 : Anti-Calmodulin antibody [EP799Y] (ab45689) at 10000 µg

    Lane 1 : RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
    Lane 2 : RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 17 kDa

    Blocking and diluting buffer: 5% NFDM/TBST

  • All lanes : Anti-Calmodulin antibody [EP799Y] (ab45689) at 1/10000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : HCT 116 (human colorectal carcinoma) whole cell lysate

    Blocking peptides at 15 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 17 kDa

    Blocking and Diluting buffer: 5% NFDM/TBST

  • ab45689 staining Calmodulin in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% Serum for 60 minutes at 4°C. Samples were incubated with primary antibody (1/100 in PBS + 0.1% Triton) for 12 hours at 4°C. A Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Anti-Calmodulin antibody [EP799Y] (ab45689) at 1/5000 dilution + NIH 3T3 cell lysate (10ug/lane)

    Predicted band size : 17 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
  • Ab45689 (1:250) staining human Calmodulin in human urinary bladder carcinoma by immunohistochemistry in paraffin embedded tissue.
  • ICC/IF image of ab45689 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45689, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing MCF-7 cells stained with ab45689 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45689, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-Calmodulin antibody [EP799Y] (ab45689) 使用論文

This product has been referenced in:
  • Wahlquist C  et al. Inhibition of miR-25 improves cardiac contractility in the failing heart. Nature 508:531-5 (2014). Read more (PubMed: 24670661) »
  • Zeng HL  et al. Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome. J Proteomics 109C:125-142 (2014). Mouse . Read more (PubMed: 24998433) »

See all 13 Publications for this product

Product Wall

Application ELISA
Sample Human Recombinant protein (Human Calmodulin full length protein)
Specification Human Calmodulin full length protein
Type Sandwich (Capture)
Username

Mr. Chang Su Jeon

Verified customer

投稿 Jul 27 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (heart)
Specification heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Nov 13 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Oct 31 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Rat Tissue sections (Muscle)
Specification Muscle
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Oct 31 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (cardiomyocytes)
Specification cardiomyocytes
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Oct 31 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Mouse Cell (cardiomyocytes)
Specification cardiomyocytes
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Oct 31 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HeLa)
Loading amount 20 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Mar 18 2013

Vielen Dank für Ihre Anfrage.

Da Abcam über 100.000 Produkte im Angebot hat ist es uns leider logistisch nicht möglich Proben Größen auf Lager zu halten und zu versenden.

Ich habe mir unsere Antikörper g...

Read More

Muchas gracias por mandarnos el cuestionario y las imágenes. Siento mucho los problemas sufridos con este anticuerpo.

He estudiado el protocolo enviado, y hay una serie de sugerencias que me gustaría haceros para intentar mejora...

Read More

1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"