製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Calmodulin
  • 特異性
    Targets C terminusof Calmodulin.
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (10-30 aa in length) conjugated to KLH and derived from the last C terminal 50 amino acids of Calmodulin (Human)

  • ポジティブ・コントロール
    • Ramos cell lysate; Human breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
  • 配列類似性
    Belongs to the calmodulin family.
    Contains 4 EF-hand domains.
  • 翻訳後修飾
    Ubiquitination results in a strongly decreased activity.
    Phosphorylation results in a decreased activity.
  • 細胞内局在
    Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
  • 別名
    • CALM 1 antibody
    • CALM 2 antibody
    • CALM 3 antibody
    • CALM antibody
    • CALM_HUMAN antibody
    • CALM1 antibody
    • CALM2 antibody
    • Calm3 antibody
    • CALML2 antibody
    • calmodulin 1 (phosphorylase kinase, delta) antibody
    • Calmodulin 2 (phosphorylase kinase, delta) antibody
    • Calmodulin 3 (phosphorylase kinase, delta) antibody
    • Calmodulin antibody
    • CaM antibody
    • CAM I antibody
    • CAM1 antibody
    • CAM2 antibody
    • CAM3 antibody
    • CAMB antibody
    • CAMC antibody
    • CAMI antibody
    • CAMII antibody
    • CPVT4 antibody
    • DD132 antibody
    • FLJ99410 antibody
    • LP7057 protein antibody
    • PHKD antibody
    • PHKD2 antibody
    • PHKD3 antibody
    • phosphorylase kinase delta antibody
    • phosphorylase kinase, delta subunit antibody
    see all

画像

  • Anti-Calmodulin antibody (ab38590) + Ramos cell lysate

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 25 kDa,30 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed paraffin-embedded human cancer tissue (breast carcinoma) with ab38590 anti-calmodulin, peroxidase-conjugated to the secondary antibody, followed by AEC staining.

参考文献

This product has been referenced in:
  • Miyoshi N  et al. Nodes-and-connections RNAi knockdown screening: identification of a signaling molecule network involved in fulvestrant action and breast cancer prognosis. Oncogenesis 4:e172 (2015). IHC . Read more (PubMed: 26479444) »
  • Hoedt E  et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . Read more (PubMed: 25116916) »

See all 3 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (sarcoma)
Specification
sarcoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6, 20 min, 100C
Permeabilization
No
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投稿 Dec 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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