Anti-Caldesmon (phospho T730) 抗体 [EPR2210] (ab75758)

製品の概要

  • 製品名Anti-Caldesmon (phospho T730) antibody [EPR2210]
    Caldesmon 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2210] to Caldesmon (phospho T730)
  • 特異性ab75758 only detects Caldesmon phosphorylated on Threonine 730.
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho-specific peptide corresponding to residues surrounding Threonine 730 of human Caldesmon

  • ポジティブ・コントロール
    • HeLa cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
IP 1/10.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IHC-P.
  • ターゲット情報

    • 機能Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
    • 組織特異性High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
    • 配列類似性Belongs to the caldesmon family.
    • ドメインThe N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
    • 翻訳後修飾In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
    • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
    • Information by UniProt
    • 参照データベース
    • 別名
      • CAD antibody
      • CALD 1 antibody
      • CALD1 antibody
      • CALD1_HUMAN antibody
      • Caldesmon 1 antibody
      • Caldesmon 1 Isoform 1 antibody
      • Caldesmon 1 Isoform 2 antibody
      • Caldesmon 1 Isoform 3 antibody
      • Caldesmon 1 Isoform 4 antibody
      • Caldesmon 1 Isoform 5 antibody
      • Caldesmon antibody
      • Caldesmon1 antibody
      • CDM antibody
      • H CAD antibody
      • HCAD antibody
      • L CAD antibody
      • LCAD antibody
      • MGC21352 antibody
      • NAG22 antibody
      see all

    Anti-Caldesmon (phospho T730) antibody [EPR2210] 画像

    • All lanes : Anti-Caldesmon (phospho T730) antibody [EPR2210] (ab75758) at 1/10000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 93 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    Anti-Caldesmon (phospho T730) antibody [EPR2210] (ab75758) 使用論文

    ab75758 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"