製品の概要

  • 製品名Anti-Caldesmon antibody [EP789Y]
    Caldesmon 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP789Y] to Caldesmon
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N-term of human Caldesmon.

  • ポジティブ・コントロール
    • HeLa cell lysate, and human normal prostate tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 93 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/50.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
    • 組織特異性High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
    • 配列類似性Belongs to the caldesmon family.
    • ドメインThe N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
    • 翻訳後修飾In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
    • 細胞内局在Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
    • Information by UniProt
    • 参照データベース
    • 別名
      • CAD antibody
      • CALD 1 antibody
      • CALD1 antibody
      • CALD1_HUMAN antibody
      • Caldesmon 1 antibody
      • Caldesmon 1 Isoform 1 antibody
      • Caldesmon 1 Isoform 2 antibody
      • Caldesmon 1 Isoform 3 antibody
      • Caldesmon 1 Isoform 4 antibody
      • Caldesmon 1 Isoform 5 antibody
      • Caldesmon antibody
      • Caldesmon1 antibody
      • CDM antibody
      • H CAD antibody
      • HCAD antibody
      • L CAD antibody
      • LCAD antibody
      • MGC21352 antibody
      • NAG22 antibody
      see all

    Anti-Caldesmon antibody [EP789Y] 画像

    • Ab45691 (1:250) staining human Caldesmon in human normal prostate tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of HeLa cells using ab45691 (1:250).
    • Anti-Caldesmon antibody [EP789Y] (ab45691) at 1/10000 dilution + HeLa cell lysate (10ug/lane)

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 93 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    Anti-Caldesmon antibody [EP789Y] (ab45691) 使用論文

    This product has been referenced in:
    • Pieretti AC  et al. A novel in vitro model to study alveologenesis. Am J Respir Cell Mol Biol 50:459-69 (2014). IHC-P . Read more (PubMed: 24066869) »
    • Sun Q  et al. Role of myocyte enhancing factor 2B in epithelial myofibroblast transition of human gingival keratinocytes. Exp Biol Med (Maywood) 237:178-85 (2012). Read more (PubMed: 22302709) »

    See all 2 Publications for this product

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