製品の概要

  • 製品名
    Anti-Caldesmon antibody [EP789Y]
    Caldesmon 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP789Y] to Caldesmon
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N-term of human Caldesmon.

  • ポジティブ・コントロール
    • HeLa cell lysate, and human normal prostate tissue.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab45691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 93 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/50.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
  • 組織特異性
    High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
  • 配列類似性
    Belongs to the caldesmon family.
  • ドメイン
    The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
  • 翻訳後修飾
    In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAD antibody
    • CALD 1 antibody
    • CALD1 antibody
    • CALD1_HUMAN antibody
    • Caldesmon 1 antibody
    • Caldesmon 1 Isoform 1 antibody
    • Caldesmon 1 Isoform 2 antibody
    • Caldesmon 1 Isoform 3 antibody
    • Caldesmon 1 Isoform 4 antibody
    • Caldesmon 1 Isoform 5 antibody
    • Caldesmon antibody
    • Caldesmon1 antibody
    • CDM antibody
    • H CAD antibody
    • HCAD antibody
    • L CAD antibody
    • LCAD antibody
    • MGC21352 antibody
    • NAG22 antibody
    see all

画像

  • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caldesmon with unpurified ab45691 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Ab45691 (1:250) staining human Caldesmon in human normal prostate tissue by immunohistochemistry using paraffin embedded tissue.
  • Immunofluorescent staining of HeLa cells using ab45691 (1:250).
  • Anti-Caldesmon antibody [EP789Y] (ab45691) at 1/10000 dilution + HeLa cell lysate (10ug/lane)

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 93 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

参考文献

This product has been referenced in:
  • Pieretti AC  et al. A novel in vitro model to study alveologenesis. Am J Respir Cell Mol Biol 50:459-69 (2014). IHC-P . Read more (PubMed: 24066869) »
  • Sun Q  et al. Role of myocyte enhancing factor 2B in epithelial myofibroblast transition of human gingival keratinocytes. Exp Biol Med (Maywood) 237:178-85 (2012). Read more (PubMed: 22302709) »

See all 2 Publications for this product

レビューと Q&A

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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