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ADDDYGRPGIEKFREEAEERDI, corresponding to amino acids 214-235 of Human Calcium Sensing Receptor.
Our Abpromise guarantee covers the use of ab19347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 130, 150 kDa.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19056349|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 2 µg/ml.|
ab19347 at 1/100 staining rat brain (cerebral cortex) tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum before incubation with the primary antibody for 24 hours at 4°C. A biotinylated horse anti-mouse IgG was used as the secondary.