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ab112115 provides a simple method for detecting calcium in physiology solutions by using our proprietary red fluorescence probe. The fluorescence signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The assay can be completed within 30 minutes. With ab112115, we have detected as little as 0.03 mM calcium. The kit has a broad dynamic range (30 µM to 1 mM).
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This product is intended to be used in vitro with solution-based samples such as cell extracts. If you would like to detect calcium in vivo using live cells, please check our Fluo-8, Rhod-4 or Fura-2 based assays.
|300 mM Calcium Standard||1 x 0.5ml|
|Assay Buffer||1 x 10ml|
|Rhod Red Indicator||2 vials|
Our Abpromise guarantee covers the use of ab112115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Ca measured in biologicals showing concentration (mM)
Standard curve (fluorimetric): mean of duplicates (+/-SD) with background readings subtracted
ab112115 has not yet been referenced specifically in any publications.