製品の概要

  • 製品名Anti-Calcium Pump PMCA3 ATPase antibody
    Calcium Pump PMCA3 ATPase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Calcium Pump PMCA3 ATPase
  • 特異性Detects PMCA 3a and 3b ATPase.
  • アプリケーション適用あり: IHC-Fr, IP, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig, Non Human Primates
  • 免疫原

    Synthetic peptide corresponding to Rat Calcium Pump PMCA3 ATPase aa 5-22.
    Sequence:

    ANSSIEFHPKPQQQREVP

  • ポジティブ・コントロール
    • WB: rat brain microsomal fractions.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/20.
IP Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 127 kDa (predicted molecular weight: 134 kDa).

This antibody detects an ~127 kDa protein representing PMCA 3a and PMCA 3b ATPase from rat brain microsomal fractions.

ELISA Use at an assay dependent concentration.
IHC-P 1/20.

ターゲット情報

  • 関連性Calcium Pump PMCA3 ATPase belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. Calcium Pump PMCA3 ATPase is the plasma membrane calcium ATPase isoform 3.
  • 細胞内局在Cell membrane; Multi-pass membrane protein.
  • 参照データベース
  • 別名
    • ATP2B3 antibody
    • ATP2B3 protein antibody
    • ATPase Ca++ transporting plasma membrane 3 antibody
    • Plasma membrane calcium ATPase 3 antibody
    • Plasma membrane calcium ATPase isoform 3 antibody
    • Plasma membrane calcium pump isoform 3 antibody
    • Plasma membrane calcium transporting ATPase 3 antibody
    • PMCA 3 antibody
    • PMCA3 antibody
    • PMCA3a antibody
    see all

Anti-Calcium Pump PMCA3 ATPase antibody 画像

  • ab3530 at 1/200 staining rat hippocampal slices and slice cultures by IHC-P. The tissue was paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with BSA before incubating with the antibody for 12 hours. An Alexa Fluor ®488 conjugated goat anti rabbit antibody was used secondary.

    See Abreview

  • All lanes : Anti-Calcium Pump PMCA3 ATPase antibody (ab3530) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from rat primary cortical neurons
    Lane 2 : Whole cell lysate prepared from rat primary cortical neurons
    Lane 3 : Whole cell lysate prepared from rat primary cortical neurons
    Lane 4 : Whole cell lysate prepared from rat primary cortical neurons

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated secondary at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 134 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 seconds

    Image courtesy of an anonymous Abreview.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA3 ATPase ab3530 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-Calcium Pump PMCA3 ATPase antibody (ab3530) 使用論文

This product has been referenced in:
  • Kosiorek M  et al. Interaction of plasma membrane Ca(2+)-ATPase isoform 4 with calcineurin A: Implications for catecholamine secretion by PC12 cells. Biochem Biophys Res Commun 411:235-40 (2011). WB ; Rat . Read more (PubMed: 21740891) »
  • Jensen TP  et al. Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic transmission in rat hippocampal CA3. J Physiol 579:85-99 (2007). WB ; Rat . Read more (PubMed: 17170045) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (primary cortical neurons)
Loading amount 20 µg
Specification primary cortical neurons
Gel Running Conditions Reduced Denaturing
Username

Abcam user community

Verified customer

投稿 Jan 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Rat hippocampal slices and slice cultures)
Specification Rat hippocampal slices and slice cultures
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 1% Triton X-100
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Dr. R Empson

Verified customer

投稿 Aug 09 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Rat forebrain or cerebellar homogenates)
Specification Rat forebrain or cerebellar homogenates
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Aug 17 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"