Anti-Calcium channel L type DHPR alpha 2 subunit 抗体 (ab62814)

製品の概要

  • 製品名
    Anti-Calcium channel L type DHPR alpha 2 subunit antibody
    Calcium channel L type DHPR alpha 2 subunit 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Calcium channel L type DHPR alpha 2 subunit
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Cow, Dog, Pig非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    C-QEIFNKYNKDKKVR

    , corresponding to internal sequence amino acids 370-383 of Human Calcium channel L type DHPR alpha 2 subunit

  • ポジティブ・コントロール
    • Human Brain (Cerebellum) lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab62814 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    Peptide ELISA: 1/16000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 123 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling.
    • 組織特異性
      Isoform 1 is expressed in skeletal muscle. Isoform 2 is expressed in the central nervous system. Isoform 2, isoform 4 and isoform 5 are expressed in neuroblastoma cells. Isoform 3, isoform 4 and isoform 5 are expressed in the aorta.
    • 配列類似性
      Belongs to the calcium channel subunit alpha-2/delta family.
      Contains 1 cache domain.
      Contains 1 VWFA domain.
    • ドメイン
      The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.
    • 翻訳後修飾
      Proteolytically processed into subunits alpha-2-1 and delta-1 that are disulfide-linked.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CA2D1_HUMAN antibody
      • CACN A2 antibody
      • CACNA2 antibody
      • Cacna2d1 antibody
      • CACNL2A antibody
      • Calcium channel L type alpha 2 polypeptide antibody
      • Calcium channel voltage dependent alpha 2/delta subunit 1 antibody
      • CCHL2A antibody
      • Dihydropyridine receptor alpha 2 subunit antibody
      • Dihydropyridine sensitive L type calcium channel alpha 2/delta subunit antibody
      • Dihydropyridine sensitive L type calcium channel subunits alpha 2/delta antibody
      • L type calcium channel subunit alpha 2 antibody
      • MHS 3 antibody
      • MHS3 antibody
      • Voltage dependent calcium channel subunit alpha 2/delta 1 antibody
      • Voltage gated calcium channel subunit alpha 2/delta 1 antibody
      • Voltage-dependent calcium channel subunit delta-1 antibody
      • Voltage-gated calcium channel subunit alpha-2/delta-1 antibody
      see all

    画像

    • Anti-Calcium channel L type DHPR alpha 2 subunit antibody (ab62814) at 0.03 µg/ml + Human Brain (Cerebellum) lysate (in RIPA buffer) at 35 µg

      Predicted band size : 123 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    This product has been referenced in:
    • Lugenbiel P  et al. Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins. PLoS One 10:e0116395 (2015). WB . Read more (PubMed: 25775120) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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