The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.
Belongs to the G-protein coupled receptor 2 family.
Rat Calcitonin receptor precursor has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data), explaining the bands seen around 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.