Anti-Calcitonin receptor/CT-R 抗体 (ab83297)
Key features and details
- Rabbit polyclonal to Calcitonin receptor/CT-R
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Calcitonin receptor/CT-R antibody
Calcitonin receptor/CT-R 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calcitonin receptor/CT-R -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following tissue lysates: Mouse small intestine; Rat kidney.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab83297の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa). |
ターゲット情報
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機能
This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin. -
配列類似性
Belongs to the G-protein coupled receptor 2 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12311 Mouse
- Entrez Gene: 116506 Rat
- SwissProt: Q60755 Mouse
- SwissProt: P32214 Rat
- Unigene: 4642 Mouse
- Unigene: 10062 Rat
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別名
- Calcitonin receptor antibody
- Calcitonin receptor precursor antibody
- CALCR antibody
see all
画像
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All lanes : Anti-Calcitonin receptor/CT-R antibody (ab83297) at 1 µg/ml
Lane 1 : Small Intestine (Mouse) Tissue Lysate
Lane 2 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Rat Calcitonin receptor precursor has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data), explaining the bands seen around 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab83297 は 1 報の論文で使用されています。
- Hughes S et al. Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina. Sci Rep 7:46085 (2017). IHC-Fr ; Mouse . PubMed: 28443635