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Our Abpromise guarantee covers the use of ab11042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/10 - 1/50.|
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||1/10 - 1/50. We recommend the use of 4% paraformaldehyde fixation, rather than formalin fixation. A sensitive detection system is also recommended. e.g. A catalysed signal amplification system. This product does not require protein digestion pre-treatment This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 56.9 kDa.|
ab11042 staining Calcitonin receptor in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton-X, blocked with 5% serum for 45 minutes at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 18 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody