Anti-Calcineurin A 抗体 [EPR1670(2)] (ab109412)

製品の概要

  • 製品名Anti-Calcineurin A antibody [EPR1670(2)]
    Calcineurin A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1670(2)] to Calcineurin A
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of Human Calcineurin A

  • ポジティブ・コントロール
    • Fetal brain, SH-SY5Y, A431, and HeLa cell lysates; HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
    • 配列類似性Belongs to the PPP phosphatase family. PP-2B subfamily.
    • 細胞内局在Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Alpha isoform formerly PPP2B antibody
      • Calcineurin A alpha antibody
      • Calcineurin A1 antibody
      • CalcineurinA antibody
      • Calmodulin dependent calcineurin A subunit alpha isoform antibody
      • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
      • CALN antibody
      • CALNA 1 antibody
      • CALNA antibody
      • CALNA1 antibody
      • CAM PRP catalytic subunit antibody
      • CAM-PRP catalytic subunit antibody
      • CCN 1 antibody
      • CCN1 antibody
      • CNA 1 antibody
      • CNA alpha antibody
      • CNA antibody
      • CNA1 antibody
      • PP2BA_HUMAN antibody
      • PPP2B antibody
      • Ppp3ca antibody
      • Protein phosphatase 2B catalytic subunit antibody
      • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
      • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
      • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
      • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
      • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
      see all

    Anti-Calcineurin A antibody [EPR1670(2)] 画像



    • Predicted band size : 59 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

      Lane 1: Wild-type HAP1 cell lysate (20 µg)

      Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)

      Lane 3: A431 cell lysate (20 µg)

      Lane 4: HeLa cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109412 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109412 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab109412 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Overlay histogram showing HeLa cells stained with ab109412 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109412, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/10000 dilution

      Lane 1 : fetal brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : A431 lysate
      Lane 4 : HeLa cells lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 59 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using 1/100 ab109412.

    Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) 使用論文

    ab109412 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109412.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"