Anti-Calcineurin A 抗体 (ab71149)
Key features and details
- Rabbit polyclonal to Calcineurin A
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Calcineurin A antibody
Calcineurin A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calcineurin A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
免疫原
A synthetic peptide corresponding to a region between residue 470 and the C-terminus (residue 521) of human Calcineurin A (NP_000935.1).
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ポジティブ・コントロール
- Whole cell lysate from hela cells, NIH3T3 cells and 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71149の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. -
配列類似性
Belongs to the PPP phosphatase family. PP-2B subfamily. -
細胞内局在
Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. - Information by UniProt
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参照データベース
- Entrez Gene: 286852 Cow
- Entrez Gene: 5530 Human
- Entrez Gene: 19055 Mouse
- Entrez Gene: 397241 Pig
- Entrez Gene: 24674 Rat
- Entrez Gene: 100144934 Xenopus tropicalis
- Omim: 114105 Human
- SwissProt: P48452 Cow
see all -
別名
- Alpha isoform formerly PPP2B antibody
- Calcineurin A alpha antibody
- Calcineurin A1 antibody
see all
画像
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All lanes : Anti-Calcineurin A antibody (ab71149) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg
Predicted band size: 59 kDa
Observed band size: 59 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Calcineurin A with ab71149 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation/ Western Blot of Calcineurin A
Lane 1: ab71149 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Subsequent WB detection was performed with 1 µg/ml ab71149.
Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab71149 は 7 報の論文で使用されています。
- Nguyen NUN et al. A calcineurin-Hoxb13 axis regulates growth mode of mammalian cardiomyocytes. Nature 582:271-276 (2020). PubMed: 32499640
- Zhang Y et al. USP16-mediated deubiquitination of calcineurin A controls peripheral T cell maintenance. J Clin Invest 129:2856-2871 (2019). PubMed: 31135381
- Tang Z et al. Quantitative Proteomic Analysis and Evaluation of the Potential Prognostic Biomarkers in Cholangiocarcinoma. J Cancer 10:3985-3999 (2019). PubMed: 31417643
- Martina JA & Puertollano R Protein phosphatase 2A stimulates activation of TFEB and TFE3 transcription factors in response to oxidative stress. J Biol Chem 293:12525-12534 (2018). PubMed: 29945972
- Haryuna TS et al. Curcumin Reduces the Noise-Exposed Cochlear Fibroblasts Apoptosis. Int Arch Otorhinolaryngol 20:370-376 (2016). IHC ; Rat . PubMed: 27746842
- Tao J et al. Endoplasmic Reticulum Stress Predicts Clinical Response to Cyclosporine Treatment in Primary Membranous Nephropathy. Am J Nephrol 43:348-56 (2016). PubMed: 27166157
- Thoms KM et al. Cyclosporin A, but not everolimus, inhibits DNA repair mediated by calcineurin: implications for tumorigenesis under immunosuppression. Exp Dermatol 20:232-6 (2011). WB ; Human . PubMed: 21323745