Anti-Calcineurin A 抗体 (ab3673)
Key features and details
- Rabbit polyclonal to Calcineurin A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
製品の概要
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製品名
Anti-Calcineurin A antibody
Calcineurin A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calcineurin A -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human Calcineurin A.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3673の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/2000 - 1/5000. Detects a band of approximately 62 kDa.
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特記事項 |
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WB
1/2000 - 1/5000. Detects a band of approximately 62 kDa. |
ターゲット情報
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機能
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. -
配列類似性
Belongs to the PPP phosphatase family. PP-2B subfamily. -
細胞内局在
Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. - Information by UniProt
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参照データベース
- Entrez Gene: 5530 Human
- Entrez Gene: 19055 Mouse
- Entrez Gene: 24674 Rat
- Omim: 114105 Human
- SwissProt: Q08209 Human
- SwissProt: P63328 Mouse
- SwissProt: P63329 Rat
- Unigene: 435512 Human
see all -
別名
- Alpha isoform formerly PPP2B antibody
- Calcineurin A alpha antibody
- Calcineurin A1 antibody
see all
画像
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All lanes : Anti-Calcineurin A antibody (ab3673) at 1/200 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 62 kDa why is the actual band size different from the predicted? -
Western Blot of wild type mouse brain tissue homogenate with ab3673 used at 1/6000. Please note that this image was assembled from two different blots. (The numbers on the scale correspond to kD).
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Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution + Huh7 (Human hepatocellular carcinoma) whole cell lysate at 15000 cells
Secondary
HRP conjugated sheep polyclonal to rabbit IgG at 1/15000 dilution
Performed under reducing conditions.
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab3673 は 21 報の論文で使用されています。
- Mitra S et al. A role for the endocannabinoid enzymes monoacylglycerol and diacylglycerol lipases in cue-induced cocaine craving following prolonged abstinence. Addict Biol 26:e13007 (2021). PubMed: 33496035
- Sánchez-Solano A et al. Regulation of the Ca2+-activated chloride channel Anoctamin-1 (TMEM16A) by Ca2+-induced interaction with FKBP12 and calcineurin. Cell Calcium 89:102211 (2020). PubMed: 32422433
- Bella P et al. Blockade of IGF2R improves muscle regeneration and ameliorates Duchenne muscular dystrophy. EMBO Mol Med 12:e11019 (2020). PubMed: 31793167
- Zheng J et al. Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway. Eur J Pharmacol 883:173386 (2020). PubMed: 32712088
- Chen X et al. Adropin regulates hepatic glucose production via PP2A/AMPK pathway in insulin-resistant hepatocytes. FASEB J 34:10056-10072 (2020). PubMed: 32579277