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Purified bovine kidney calbindin-D-28K
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab108404 as a replacement.
Our Abpromise guarantee covers the use of ab82812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20542987|
|IHC-P||1/15 - 1/30. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Immunohistochemical analysis of FFPE human kidney tissue, labeling Calbindin with ab82812.
ab82812 staining rat brain (corpus callosum) sections by IHC-FoFr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected with 30% sucrose and sectioned using a cryostat. Staining with ab82812 at a 1/2800 dilution in PBS-Triton (0.3%) with 0.02% azide was performed for 18h at 25°C. A donkey anti-mouse Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"