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Purified bovine kidney calbindin-D-28K
Our Abpromise guarantee covers the use of ab82812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20542987|
|IHC-P||1/15 - 1/30. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab82812 staining rat brain (corpus callosum) sections by IHC-FoFr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected with 30% sucrose and sectioned using a cryostat. Staining with ab82812 at a 1/2800 dilution in PBS-Triton (0.3%) with 0.02% azide was performed for 18h at 25°C. A donkey anti-mouse Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody.
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