Anti-Calcineurin inhibitor 抗体 (ab3349)
Key features and details
- Rabbit polyclonal to Calcineurin inhibitor
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
製品の概要
-
製品名
Anti-Calcineurin inhibitor antibody -
製品の詳細
Rabbit polyclonal to Calcineurin inhibitor -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide (His-tag) corresponding to Rat Calcineurin inhibitor aa 1850 to the C-terminus.
Database link: O88480 -
ポジティブ・コントロール
- ICC/IF: PC12 and NRK cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: 99% PBS -
Concentration information loading...
-
精製度
Whole antiserum -
一次抗体 備考
Calcineurin is a widely distributed Ser/Thr, calcium and calmodulin-dependent protein phosphatase. It mediates the immunosuppressive actions of drugs such as FK506 and cyclosporin, and has also been implicated in a number of calcium-sensitive pathways in the nervous system. Calcineurin has been found to be associated with other proteins, such as calmodulin, FKBP12, the ryanodine receptor, inositol 1,4,5-trisphosphate, and a recently identified protein, calcineurin inhibitor (CAIN). CAIN has been shown to bind calcineurin through its C-terminal tail. Recent studies have shown CAIN to bind to both amphiphysin 1 and calcineurin simultaneously and therefore acts as a physiological inhibitor of calcineurin. The binding of CAIN to amphiphysin 1 does not affect the interaction of amphiphysin 1 with other endocytic proteins. CAIN, like calcineurin, is highly expressed in neurons and other tissues. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3349の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/1000.
|
特記事項 |
---|
ICC/IF
1/1000. |
ターゲット情報
-
機能
May be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals. -
組織特異性
Widely expressed in different tissues. -
配列類似性
Contains 6 TPR repeats. -
翻訳後修飾
Activated through PKC-mediated hyperphosphorylation. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 94165 Rat
- SwissProt: O88480 Rat
- Unigene: 4 Rat
-
別名
- A330070M20Rik antibody
- Cabin 1 antibody
- CABIN_HUMAN antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence analysis of NRK cells labeling Calcineurin inhibitor with ab3349. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with ab3349 at a dilution of 20 µg/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate at a dilution of 1/1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye.
-
ICC/IF image of ab3349 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3349, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab3349 は 9 報の論文で使用されています。
- Smith R et al. HIRA contributes to zygote formation in mice and is implicated in human 1PN zygote phenotype. Reproduction 161:697-707 (2021). PubMed: 33835048
- Hoang SM et al. Regulation of ALT-associated homology-directed repair by polyADP-ribosylation. Nat Struct Mol Biol 27:1152-1164 (2020). PubMed: 33046907
- Torné J et al. Two HIRA-dependent pathways mediate H3.3 de novo deposition and recycling during transcription. Nat Struct Mol Biol 27:1057-1068 (2020). PubMed: 32895554
- Zhang L et al. Downregulation of miRNA-15a and miRNA-16 promote tumor proliferation in multiple myeloma by increasing CABIN1 expression. Oncol Lett 15:1287-1296 (2018). PubMed: 29399181
- Ray-Gallet D et al. Functional activity of the H3.3 histone chaperone complex HIRA requires trimerization of the HIRA subunit. Nat Commun 9:3103 (2018). PubMed: 30082790
- Lee JS & Zhang Z O-linked N-acetylglucosamine transferase (OGT) interacts with the histone chaperone HIRA complex and regulates nucleosome assembly and cellular senescence. Proc Natl Acad Sci U S A 113:E3213-20 (2016). PubMed: 27217568
- Ying CY et al. MEF2B mutations lead to deregulated expression of the oncogene BCL6 in diffuse large B cell lymphoma. Nat Immunol 14:1084-92 (2013). PubMed: 23974956
- Rai TS et al. Human CABIN1 is a functional member of the human HIRA/UBN1/ASF1a histone H3.3 chaperone complex. Mol Cell Biol 31:4107-18 (2011). WB, ICC/IF, IP ; Human . PubMed: 21807893
- Elsaesser SJ & Allis CD HIRA and Daxx constitute two independent histone H3.3-containing predeposition complexes. Cold Spring Harb Symp Quant Biol 75:27-34 (2010). WB . PubMed: 21047901