製品の概要

  • 製品名Anti-Cadherin 16 antibody
    Cadherin 16 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cadherin 16
  • アプリケーション適用あり: IHC-P, ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Orangutan
  • 免疫原

    Synthetic peptide: PFLFLEASDR DEPGTANSDL RFHILSQAPA QPSPDMFQLE PRLGALALSP, corresponding to amino acids 144-193 of Human Cadherin 16 (NP_004053).

  • ポジティブ・コントロール
    • Human fetal brain lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

ターゲット情報

Anti-Cadherin 16 antibody 画像

  • Anti-Cadherin 16 antibody (ab80320) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 90 kDa
    Observed band size : 92 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • ab80320 staining Cadherin 16 human kidney cells (RPTEC/TERT1) by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol, blocked using 5 % BSA, 1 % Triton X-100 for 1 hour at 25°C and then incubated with ab80320 at a 1/100 dilution for 14 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-rabbit polyclonal used at a 1/800 dilution.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cadherin 16 with ab80320 at 5µg/ml.

Anti-Cadherin 16 antibody (ab80320) 使用論文

This product has been referenced in:
  • Aschauer L  et al. Delineation of the key aspects in the regulation of epithelial monolayer formation. Mol Cell Biol 33:2535-50 (2013). Read more (PubMed: 23608536) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Cow Tissue lysate - whole (Kidney)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 10 µg
Specification Kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Verified customer

投稿 Oct 07 2016

Thank you for contacting us.

I have added the code and published your review. We are happy that this product worked well for you in this application. Thank you very much for participating in our 100% Abreview testing program.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Kidney (RPTEC/TERT1))
Specification Kidney (RPTEC/TERT1)
Fixative Methanol
Permeabilization No
Blocking step 5 % BSA, 1 % Triton X-100 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 15 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"