製品の概要

  • 製品名Anti-CAD antibody
    CAD 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CAD
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human CAD (NP_004332.2).

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
  • 細胞内局在Cytoplasmic and Nuclear
  • 参照データベース
  • 別名
    • Aspartate carbamoyltransferase antibody
    • Aspartate transcarbamylase antibody
    • CAD protein antibody
    • CAD trifunctional protein antibody
    • Carbamoyl phosphate synthetase 2 antibody
    • CPSase ATCase DHOase antibody
    • Dihydroorotase antibody
    • Glutamine dependent carbamoyl phosphate synthase antibody
    • Multifunctional protein CAD antibody
    see all

Anti-CAD antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CAD with ab99311 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CAD antibody (ab99311) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 243 kDa


    Exposure time : 10 seconds
  • Detection of CAD by Western Blot of Immunprecipitate.
    ab99311 at 0.1µg/ml staining CAD in HeLa whole cell lysate immunoprecipitated using ab99311 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-CAD antibody (ab99311) 使用論文

ab99311 has not yet been referenced specifically in any publications.

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