製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CACNG4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • 免疫原

    Synthetic peptide: IEGIYKGHCF RINHFPEDND YDHDSSEYLL RIVRASSVFP ILSTILLLLG , corresponding to N terminal amino acids 73-122 of Human CACNG4

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state.
  • 配列類似性
    Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CACNG4 antibody
    • Calcium channel voltage dependent gamma subunit 4 antibody
    • CCG4_HUMAN antibody
    • Neuronal voltage gated calcium channel gamma 4 subunit antibody
    • Neuronal voltage-gated calcium channel gamma-4 subunit antibody
    • TARP gamma-4 antibody
    • Transmembrane AMPAR regulatory protein gamma-4 antibody
    • Voltage dependent calcium channel gamma 4 subunit antibody
    • Voltage-dependent calcium channel gamma-4 subunit antibody
    see all

画像

  • Anti-CACNG4 antibody (ab80381) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa

プロトコール

参考文献

ab80381 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (HeLa cells = -/+ transfected with CACNG4 ms frog o)
Gel Running Conditions
Reduced Denaturing (12 % gel)
Loading amount
15 µg
Specification
HeLa cells = -/+ transfected with CACNG4 ms frog o
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jul 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録