製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CACNG1
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    A region within synthetic peptide: SKTCGPITLP GEKNCSYFRH FNPGESSEIF EFTTQKEYSI SAAAIAIFSL, corresponding to amino acids 66-115 of Human CACNG1

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab48550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    This protein is a subunit of the dihydropyridine (DHP) sensitive calcium channel. Plays a role in excitation-contraction coupling. The skeletal muscle DHP-sensitive Ca(2+) channel may function only as a multiple subunit complex.
  • 組織特異性
    Skeletal muscle.
  • 配列類似性
    Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CACNG1 antibody
    • CACNLG antibody
    • CACNLG calcium channel neuronal dihydrpyridine sensitive gamma subunit antibody
    • Calcium channel L type gamma polypeptide antibody
    • Calcium channel voltage dependent gamma subunit 1 antibody
    • CCG1_HUMAN antibody
    • Dihydropyridine sensitive L type skeletal muscle calcium channel gamma subunit antibody
    • Dihydropyridine sensitive L type skeletal muscle calcium channel subunit gamma antibody
    • Dihydropyridine-sensitive L-type antibody
    • fb43f02 antibody
    • fi19g08 antibody
    • L type calcium channel gamma polypeptide antibody
    • MGC117703 antibody
    • skeletal muscle calcium channel subunit gamma antibody
    • Voltage dependent calcium channel gamma 1 subunit 2 antibody
    • Voltage-dependent calcium channel gamma-1 subunit antibody
    • wu:fb43f02 antibody
    • zgc:55364 antibody
    see all

画像

  • Anti-CACNG1 antibody (ab48550) at 0.5 µg/ml + 10ug Jurkat cell lysate with 5% skimmed milk in PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 25 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa (possible non-specific binding),48 kDa (possible non-specific binding).

プロトコール

参考文献

ab48550 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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