製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CACNB1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide derived from residues 21 - 70 of human CACNB1.

    .

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

ターゲット情報

  • 機能
    The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
  • 組織特異性
    Isoform 1 and isoform 3 are expressed in brain, heart, spleen, central nervous system and neuroblastoma cells. Isoform 2 is expressed in skeletal muscle.
  • 配列類似性
    Belongs to the calcium channel beta subunit family.
    Contains 1 SH3 domain.
  • 細胞内局在
    Cell membrane > sarcolemma.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAB1 antibody
    • CACB1_HUMAN antibody
    • CACNB1 antibody
    • CACNLB1 antibody
    • Calcium channel L type beta 1 polypeptide antibody
    • Calcium channel voltage dependent beta 1 subunit antibody
    • Calcium channel voltage-dependent subunit beta 1 antibody
    • CCHLB1 antibody
    • Dihydropyridine sensitive L type calcium channel beta 1 subunit antibody
    • MGC41896 antibody
    • Voltage dependent L type calcium channel beta 1 subunit antibody
    • Voltage-dependent L-type calcium channel subunit beta-1 antibody
    see all

画像



  • Predicted band size : 66 kDa
    Western Blot using ab28502 at 1.0ug/ml. Sample type: Hum. Adult Placenta Lysate


  • Predicted band size : 66 kDa
    Western Blot using ab28502 at 1.0ug/ml. Sample type: Hum. Fetal Heart Lysate


  • Predicted band size : 66 kDa
    Western Blot using ab28502 at 1.0ug/ml. Sample type: Hum. Fetal Liver Lysate


  • Predicted band size : 66 kDa
    Western Blot using ab28502 at 1.0ug/ml. Sample type: Hum. Fetal Lung Lysate


  • Predicted band size : 66 kDa
    Western Blot using ab28502 at 1.0ug/ml. Sample type: Hum. Fetal Muscle Lysate
  • Anti-CACNB1 antibody (ab28502) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 66 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Gel Concentration: 12%

プロトコール

参考文献

This product has been referenced in:
  • Ruan B  et al. Binding of rapamycin analogs to calcium channels and FKBP52 contributes to their neuroprotective activities. Proc Natl Acad Sci U S A 105:33-8 (2008). WB . Read more (PubMed: 18162540) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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