製品の概要

  • 製品名Anti-CACNA1S antibody
    CACNA1S 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CACNA1S
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cat
  • 免疫原

    Recombinant fragment, corresponding to a region within the internal sequence amino acids 544-855 of Human CACNA1S.

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji cell lysates Cal27 xenograft (IHC)

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 212 kDa.
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.

ターゲット情報

  • 機能Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle.
  • 組織特異性Skeletal muscle specific.
  • 関連疾患Defects in CACNA1S are the cause of periodic paralysis hypokalemic type 1 (HOKPP1) [MIM:170400]; also designated HYPOPP. HOKPP1 is an autosomal dominant disorder manifested by episodic flaccid generalized muscle weakness associated with falls of serum potassium levels.
    Defects in CACNA1S are the cause of malignant hyperthermia susceptibility type 5 (MHS5) [MIM:601887]; an autosomal dominant disorder that is potentially lethal in susceptible individuals on exposure to commonly used inhalational anesthetics and depolarizing muscle relaxants.
    Defects in CACNA1S are the cause of susceptibility to thyrotoxic periodic paralysis type 1 (TTPP1) [MIM:188580]. A sporadic muscular disorder characterized by episodic weakness and hypokalemia during a thyrotoxic state. It is clinically similar to hereditary hypokalemic periodic paralysis, except for the fact that hyperthyroidism is an absolute requirement for disease manifestation. The disease presents with recurrent episodes of acute muscular weakness of the four extremities that vary in severity from paresis to complete paralysis. Attacks are triggered by ingestion of a high carbohydrate load or strenuous physical activity followed by a period of rest. Thyrotoxic periodic paralysis can occur in association with any cause of hyperthyroidism, but is most commonly associated with Graves disease.
  • 配列類似性Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1S subfamily.
  • ドメインEach of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    The loop between repeats II and III interacts with the ryanodine receptor, and is therefore important for calcium release from the endoplasmic reticulum necessary for muscle contraction.
  • 翻訳後修飾Phosphorylation by PKA activates the calcium channel.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • alpha-1 polypeptide antibody
    • CAC1S_HUMAN antibody
    • CACH1 antibody
    • Cach1b antibody
    • CACN1 antibody
    • CACNA1S antibody
    • CACNL1A3 antibody
    • Calcium channel antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle, hypokalemic periodic paralysis antibody
    • Calcium channel, skeletal muscle dihydropyridine sensitive , alpha 1 subunit antibody
    • Calcium channel, voltage dependent, L type, alpha 1S subunit antibody
    • Calcium channel, voltage-dependent, L type, alpha 1S subunit, b antibody
    • Cav1.1 antibody
    • CCHL1A3 antibody
    • Dihydropyridine receptor antibody
    • Dihydropyridine sensitive L type calcium channel alpha 1 subunit antibody
    • fmd antibody
    • HOKPP antibody
    • HypoPP antibody
    • isoform 3 antibody
    • L type antibody
    • Malignant hyperthermia susceptibility 5 antibody
    • mdg antibody
    • MHS5 antibody
    • ROB1 antibody
    • sj antibody
    • skeletal muscle antibody
    • TTPP1 antibody
    • Voltage gated calcium channel subunit alpha Cav1.1 antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1S antibody
    • Voltage-gated calcium channel subunit alpha Cav1.1 antibody
    see all

Anti-CACNA1S antibody 画像

  • Anti-CACNA1S antibody (ab96413) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 212 kDa
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab96413 at 1:100 dilution.

Anti-CACNA1S antibody (ab96413) 使用論文

ab96413 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12)
Loading amount 10 µg
Specification C2C12
Gel Running Conditions Reduced Denaturing (4-20% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 01 2011

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