Anti-CACNA1C 抗体 [S57] (ab84814)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [S57] to CACNA1C
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Human, Recombinant fragment
製品の概要
-
製品名
Anti-CACNA1C antibody [S57]
CACNA1C 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [S57] to CACNA1C -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-P, WB, Flow Cytmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
Fusion protein corresponding to Rabbit CACNA1C aa 1500-1750 (C terminal).
Database link: P15381 -
ポジティブ・コントロール
- WB: Cell lysates prepared from DHPR alpha 1 transfected CHO cells. IHC-P: Human hippocampus tissue; Mouse brain tissue. ICC/IF: SK-N-BE cells. Flow Cyt: SH-SY5Y cells.
-
特記事項
The clone number has been updated from S57-46 to L57/46, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
-
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
S57 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84814の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/100.
|
|
IHC-P |
Use a concentration of 0.1 - 10 µg/ml.
Use at 0.1-1.0µg/mL (perox), 1.0-10µg/mL (IF). |
|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 240 kDa.
If results are poor, use lysate without boiling, heat at 37°C for 15 minutes. |
|
Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
---|
ICC/IF
1/100. |
IHC-P
Use a concentration of 0.1 - 10 µg/ml. Use at 0.1-1.0µg/mL (perox), 1.0-10µg/mL (IF). |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 240 kDa. If results are poor, use lysate without boiling, heat at 37°C for 15 minutes. |
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
-
機能
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function. -
組織特異性
Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle. -
関連疾患
Timothy syndrome
Brugada syndrome 3 -
配列類似性
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily. -
ドメイン
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel. -
翻訳後修飾
Phosphorylation by PKA activates the channel. -
細胞内局在
Membrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 775 Human
- Entrez Gene: 12288 Mouse
- Omim: 114205 Human
- SwissProt: Q13936 Human
- SwissProt: Q01815 Mouse
- Unigene: 118262 Human
- Unigene: 690010 Human
- Unigene: 41628 Mouse
see all -
別名
- alpha-1 polypeptide antibody
- cardiac muscle antibody
- isoform 1 antibody
see all
画像
-
All lanes : Anti-CACNA1C antibody [S57] (ab84814) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from DHPR alpha 1 transfected CHO cells -
ab84814 staining CACNA1C in human hippocampus tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin embedded tissue sections). Tissue underwent heat mediate fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A Fluorophore conjugated goat anti-mouse was used as secondary at 1/50 dilution.
-
ab84814 staining CACNA1C in mouse brain tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue section. Tissue underwent heat mediated fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A HRP conjugated secondary was used at 10 dilution.
-
Overlay histogram showing SH-SY5Y cells stained with ab84814 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84814, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
-
Formaldehyde-fixed SK-N-BE cells stained for CACNA1C (green) using ab84814 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/200 dilution for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain (A); DAPI (B, blue) nuclear stain at 1/1000, 1/5000 for 60 minutes at room temperature, 5 minutes at room temperature.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (23)
ab84814 は 23 報の論文で使用されています。
- Adili A et al. Atrial Fibrillation Underlies Cardiomyocyte Senescence and Contributes to Deleterious Atrial Remodeling during Disease Progression. Aging Dis 13:298-312 (2022). PubMed: 35111375
- Jiang X et al. MCC950 ameliorates ventricular arrhythmia vulnerability induced by heart failure. Bioengineered 13:8593-8604 (2022). PubMed: 35287557
- Huang Y et al. Nisoldipine Inhibits Influenza A Virus Infection by Interfering with Virus Internalization Process. Viruses 14:N/A (2022). PubMed: 36560742
- Jiang T et al. IL-6/STAT3 Signaling Promotes Cardiac Dysfunction by Upregulating FUNDC1-Dependent Mitochondria-Associated Endoplasmic Reticulum Membranes Formation in Sepsis Mice. Front Cardiovasc Med 8:790612 (2021). PubMed: 35118141
- Song Q et al. Astragaloside IV protects against retinal iron overload toxicity through iron regulation and the inhibition of MAPKs and NF-?B activation. Toxicol Appl Pharmacol 410:115361 (2021). PubMed: 33285147