製品の概要
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製品名Anti-CACNA1B (N type) antibody
CACNA1B (N type) 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to CACNA1B (N type)
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アプリケーション適用あり: IHC-P, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Rat, Rabbit
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免疫原
2013 - 2179 of Human CACNA1B (N type) (NP_000709)
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ポジティブ･コントロール
- A549 whole cell lysate and HCT116 cell lysate IHC-P: FFPE human cerebral cortex tissue sections.
法規制情報
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 1X PBS, pH 7 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab97284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | 1/1000. Predicted molecular weight: 263 kDa. |
ターゲット情報
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関連性Voltage-sensitive calcium channels (VSCCs) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death.
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細胞内局在Membrane; Multi-pass membrane protein.
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参照データベース
- Entrez Gene: 774 Human
- Entrez Gene: 100008979 Rabbit
- Entrez Gene: 257648 Rat
- Omim: 601012 Human
- SwissProt: Q00975 Human
- SwissProt: Q05152 Rabbit
- SwissProt: Q02294 Rat
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別名
- BIII antibody
- Brain calcium channel III antibody
- CACH5 antibody
see all
Anti-CACNA1B (N type) antibody 画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1B (N type) antibody (ab97284)IHC image of CACNA1B (N type) staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97284, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - CACNA1B (N type) antibody (ab97284)Anti-CACNA1B (N type) antibody (ab97284) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size : 263 kDa
5% SDS PAGE
Anti-CACNA1B (N type) antibody (ab97284) 使用論文
ab97284 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"