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製品の概要

  • 製品名Anti-CACNA1B (N type) antibody
    CACNA1B (N type) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CACNA1B (N type)
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit
  • 免疫原

    2013 - 2179 of Human CACNA1B (N type) (NP_000709)

  • ポジティブ･コントロール
    • A549 whole cell lysate and HCT116 cell lysate IHC-P: FFPE human cerebral cortex tissue sections.

法規制情報

医薬用外毒物

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 1X PBS, pH 7
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 263 kDa.

ターゲット情報

  • 関連性Voltage-sensitive calcium channels (VSCCs) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death.
  • 細胞内局在Membrane; Multi-pass membrane protein.
  • 参照データベース
    • 別名
      • BIII antibody
      • Brain calcium channel III antibody
      • CACH5 antibody
      • CACNL1A5 antibody
      • CACNN antibody
      • Calcium channel alpha12.2 subunit antibody
      • Calcium channel L type alpha 1 polypeptide isoform 5 antibody
      • Calcium channel L type, alpha 1 polypeptide antibody
      • Calcium channel N type antibody
      • Calcium channel voltage dependent alpha 1B subunit N type antibody
      • Calcium channel voltage dependent L type alpha 1B subunit antibody
      • Calcium channel voltage dependent N type alpha 1B subunit antibody
      • Cav2.2 antibody
      • Cav2.2 voltage gated Ca2+ channel antibody
      • Voltage dependent N type calcium channel subunit alpha 1B antibody
      • Voltage gated calcium channel alpha subunit Cav2.2 antibody
      see all

    Anti-CACNA1B (N type) antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1B (N type) antibody (ab97284)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1B (N type) antibody (ab97284)

      IHC image of CACNA1B (N type) staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97284, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - CACNA1B (N type) antibody (ab97284)
      Western blot - CACNA1B (N type) antibody (ab97284)
      Anti-CACNA1B (N type) antibody (ab97284) at 1/1000 dilution + A549 whole cell lysate at 30 µg

      Predicted band size : 263 kDa
      5% SDS PAGE

    Anti-CACNA1B (N type) antibody (ab97284) 使用論文

    ab97284 has not yet been referenced specifically in any publications.

    ab97284 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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