製品の概要

  • 製品名Anti-CACNA1A antibody
    CACNA1A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CACNA1A
  • アプリケーション適用あり: WB, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Rabbit, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat CACNA1A.

    (Peptide available as ab32641.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Rat brain cerebellum, E16 rat brain, E18 rat brain, E16 mouse brain.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 190 kDa (predicted molecular weight: 268 kDa).Can be blocked with Rat CACNA1A peptide (ab32641). PubMed: 21293071

CACNA1a has a major form with an apparent molecular mass of 190 kDa and a minor, full-length form with an apparent molecular mass of 220 kDa. [PMID:7673157]

IHC-FoFr 1/100.

ターゲット情報

  • 関連性Cav2.1 is a voltage-sensitive calcium channels (VSCC) which belongs to the calcium channel alpha-1 subunit family. Cav2.1 mediates the entry of calcium ions into excitable cells and is also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. Cav2.1 (isoform alpha-1A) gives rise to P and/or Q-type calcium currents. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity.
  • 細胞内局在Cell Membrane
  • 参照データベース
  • 別名
    • APCA antibody
    • BI antibody
    • Brain calcium channel 1 antibody
    • Brain calcium channel I antibody
    • CACH4 antibody
    • CACN3 antibody
    • CACNA1A antibody
    • CACNL1A4 antibody
    • Calcium channel alpha 1A subunit antibody
    • Calcium channel L type alpha 1 polypeptide antibody
    • Calcium channel L type alpha 1 polypeptide isoform 4 antibody
    • Calcium channel voltage dependent, P/Q type alpha 1A subunit antibody
    • CAV2.1 antibody
    • EA2 antibody
    • FHM antibody
    • HPCA antibody
    • MHP antibody
    • MHP1 antibody
    • RAT brain class A antibody
    • RBA-I antibody
    • SCA6 antibody
    • Voltage dependent P/Q type calcium channel alpha 1A subunit antibody
    • Voltage gated calcium channel alpha subunit Cav2.1 antibody
    see all

Anti-CACNA1A antibody 画像

  • All lanes : Anti-CACNA1A antibody (ab32642) at 1 µg/ml

    Lane 1 : E16 Rat Embryo Brain - Tissue Lysate
    Lane 2 : E16 Mouse Embryo Brain - Tissue Lysate
    Lane 3 : E18 Rat Embryo Brain - Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 268 kDa
    Observed band size : 190 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab32642 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • Anti-CACNA1A antibody (ab32642) at 1 µg/ml + Cerebellum Rat Tissue Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 268 kDa
    Observed band size : 190 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 56 kDa. We are unsure as to the identity of these extra bands.
    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunofluorescent staining for CACNA1A using Rabbit polyclonal to CACNA1A (ab32642)in rat Purkinje (arrows) and granular (arrow heads) cells, of the rat cerebellum. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab32642 was incubated overnight at 1/3000 at room temperature. Secondary antibody Alexa fluor 488 1/3000 was incubated for 2 hours at room temperature.

Anti-CACNA1A antibody (ab32642) 使用論文

This product has been referenced in:
  • Vares G  et al. Gene silencing of Tead3 abrogates radiation-induced adaptive response in cultured mouse limb bud cells. J Radiat Res (Tokyo) 52:39-46 (2011). WB ; Mouse . Read more (PubMed: 21293071) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
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投稿 Sep 25 2014

Thank you very much for contacting us with your question.

The immunogen of ab32642 is 13 amino acids long, and 10 of these amino acids are identical to the Xenopus XP_002940668 (77% identity). There are 7 consecutive identical amino acids. Read More

Thank you for contacting us.

We currently offer two products againstCACNA1A. Ab81011 is raised against an immunogen located within the 1000-1100 amino acid range of human and has been tested inWB,IHC-P,IHC-Fr. Our other productab32642 is r...

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