製品の概要

  • 製品名Anti-CABP antibody
    CABP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CABP
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CABP.

    (Peptide available as ab108465.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse cerebellum; Mouse hippocampus; Mouse spinal cord.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab93853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

ターゲット情報

  • 機能Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs). Inhibits agonist-induced intracellular calcium signaling. Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels. Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but resulting in an opposit effects on channel function. Suppresses the calcium-dependent inactivation of CACNA1D (By similarity). Inhibits TRPC5 channels. Prevents NMDA receptor-induced cellular degeneration.
  • 組織特異性Retina and brain. Somatodendritic compartment of neurons. Calbrain was found exclusively in brain where it is abundant in the hippocampus, habenular area in the epithalamus and in the cerebellum.
  • 配列類似性Contains 4 EF-hand domains.
  • ドメインEF-1 binds magnesium constitutively under physiological conditions, EF-3 and EF-4 bind calcium cooperatively and EF-2 binds neither calcium nor magnesium.
  • 翻訳後修飾Phosphorylated. The phosphorylation regulates the activity.
  • 細胞内局在Cytoplasm > cell cortex. Cell membrane. S-CaBP1 is localized at or near the plasma membrane; Cytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Cell membrane. Golgi apparatus. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. L-CaBP1 is associated most likely with the cytoskeletal structures, whereas S-CaBP1 is localized at or near the plasma membrane and Cytoplasm > cytoskeleton. L-CaBP1 is associated most likely with the cytoskeletal structures.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CABP 1 antibody
    • CaBP1 antibody
    • CABP1_HUMAN antibody
    • Calbrain antibody
    • Calcium binding protein 1 antibody
    • Calcium binding protein 5 antibody
    • Calcium-binding protein 1 antibody
    • Caldendrin antibody
    • HCALB BR antibody
    • HCALB_BR antibody
    see all

Anti-CABP antibody 画像

  • All lanes : Anti-CABP antibody (ab93853) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 85 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-CABP antibody (ab93853) 使用論文

ab93853 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (primary hippocampal neurons)
Specification primary hippocampal neurons
Fixative Paraformaldehyde
Permeabilization Yes - ice-cold methanol (optional)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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投稿 Jun 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (primary hippocampal neurons)
Loading amount 20 µg
Specification primary hippocampal neurons
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jun 14 2012

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Der Anti-CABP-Antikörper ab93853wurde nach einem Standard-Westernblot-Protokoll getestet, das auch auf unserer Webseite zu finden ist(siehe Anhang und http://www.abcam.com/index.html?pageconfig=popular...

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