The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter.
Detected in brain neurons.
Contains 6 FF domains. Contains 3 WW domains.
The FF domains bind the phosphorylated C-terminus of the largest subunit of RNA polymerase II, probably mediate interaction with HTATSF1 and preferentially bind peptides with the consensus sequence [DE](2-5)-[FWY]-[DE](2-5). The WW domains bind Pro-rich domains.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling CA150 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-CA150 antibody (ab10481)
ab10481 detection of human CA150 by Western blot.Sample: Nuclear extract (5 µg) from HeLa cells. Antibody: ab10481 used at 0.33 µg/ml. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3,000 with NBT-BCIP as substrate for 20 min.
ab10481 detection of human CA150 by Western blot. Sample: Nuclear extract (5 µg) from HeLa cells. Antibody: ab10481 used at 0.33 µg/ml. Detection: Alkaline phosphatase conjugated goat anti-Rabbit IgG diluted 1:3,000 with NB