Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% acrylamyde)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-0,05%Tween -5%Milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit DAKO
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500
Detection
Detection method
Super Signal WestDura
Bands
Specific: 70 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
TNFalpha 250u/ml 8hrs
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 04 2013