The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
C9orf156 hydrolyzes acyl-CoA thioesters (in vitro). It has a preference for substrates with medium chain length (C10-C14) and is inactive towards substrates with C18 or C20 aliphatic chains. Its physiological function is not known. C9orf156 interacts with Nef from human immunodeficiency virus (HIV-1).