製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to C9orf135
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen, corresponding to amino acids 1-71 (MDSLDRSCQD WCDRKQHWLE IGPPDLVERK GSLTLRSHHK KYSKPVLVYS WHRDREAFPK GYDIEGPEKV K) of Human C9orf135.

  • ポジティブ・コントロール
    • Human stomach tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

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  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleoli, nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121659 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1 :
    Lanes 2 - 3 : Anti-C9orf135 antibody (ab121659)

    Lane 1 : Marker
    Lane 2 : HEK293T cell lysate - vector only transfected
    Lane 3 : HEK293T cell lysate - overexpressing C9orf135 with a C-terminal myc-DDK tag (~3.1kDa)
  • ab121659, at 1/500 dilution, staining C9orf135 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

参考文献

ab121659 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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