製品の概要

  • 製品名
    Anti-C6orf222 antibody
  • 製品の詳細
    Rabbit polyclonal to C6orf222
  • アプリケーション
    適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: SFRRAFYHKK HTSKEPRRAG AAGAASPEAR RPKRPSFLPL CVGGHRPSTS SSLDPEDLEC REPLPAEGEP VVISEAPSQ, corresponding to internal sequence amino acids 438-516 of Human C6orf222.

  • ポジティブ・コントロール
    • Human testis tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

ターゲット情報

Anti-C6orf222 antibody 画像

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122471 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122471, at 1/200 dilution, staining C6orf222 in paraffin-embedded Human testis tissue by Immunohistochemitry.

Anti-C6orf222 antibody (ab122471) 使用論文

ab122471 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (human bronchial epithelial cell, lung)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
60 µg
Specification
human bronchial epithelial cell, lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Miss. Hsin-Ju Wang

Verified customer

投稿 Dec 19 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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