製品の概要

  • 製品名Anti-C6ORF173 antibody
    C6ORF173 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C6ORF173
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to a region of Human C6ORF173

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab75827 is purified by a peptide affinity chromatography method.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab75827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation (By similarity). The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres (By similarity). Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPW has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.
  • 組織特異性Highly expressed in ovary, liver, lung and pancreas and to a lower extent in breast and gastrointestinal tract cancers; such as those of the colon, rectum and stomach. Overexpressed in high grade breast invasive tumors. Expressed in many cancer cell types.
  • 配列類似性Belongs to the CENPW family.
  • 細胞内局在Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Nucleus matrix. Nucleus > nucleolus. Constitutively localizes to centromeres throughout the cell cycle, and to the inner kinetochore during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cancer upregulated gene 2 antibody
    • Cancer-up-regulated gene 2 protein antibody
    • CENP-W antibody
    • cenpw antibody
    • CENPW_HUMAN antibody
    • Centromere protein W antibody
    • chromosome 6 open reading frame 173 antibody
    • CUG2 antibody
    • hypothetical protein LOC387103 antibody
    see all

Anti-C6ORF173 antibody 画像

  • Anti-C6ORF173 antibody (ab75827) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 10 kDa
    Observed band size : 10 kDa

Anti-C6ORF173 antibody (ab75827) 使用論文

This product has been referenced in:
  • Prendergast L  et al. Premitotic Assembly of Human CENPs -T and -W Switches Centromeric Chromatin to a Mitotic State. PLoS Biol 9:e1001082 (2011). WB ; Human . Read more (PubMed: 21695110) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"