Anti-C6 抗体 (ab74638)
Key features and details
- Rabbit polyclonal to C6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-C6 antibody
C6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to C6 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
-
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Pig -
免疫原
Synthetic peptide corresponding to Human C6 aa 200-300 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab74637) -
ポジティブ・コントロール
- This antibody gave a positive signal in Human Plasma (Total Protein)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Matched antibody pairs
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74638の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).
|
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
特記事項 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
-
機能
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. -
関連疾患
Defects in C6 are the cause of complement component 6 deficiency (C6D) [MIM:612446]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. -
配列類似性
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 2 Kazal-like domains.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 2 Sushi (CCP/SCR) domains.
Contains 3 TSP type-1 domains. -
翻訳後修飾
All cysteine residues are assumed to be cross-linked to one another. Individual modules containing an even number of conserved cysteine residues are supposed to have disulfide linkages only within the same module. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 729 Human
- Entrez Gene: 12274 Mouse
- Omim: 217050 Human
- SwissProt: P13671 Human
- Unigene: 481992 Human
-
別名
- AW111623 antibody
- C6 antibody
- CO6_HUMAN antibody
see all
画像
-
All lanes : Anti-C6 antibody (ab74638) at 1 µg/ml
Lane 1 : Human Plasma Total Protein Lysate at 2 µg
Lane 2 : Human Plasma Total Protein Lysate at 5 µg
Lane 3 : Human Plasma Total Protein Lysate at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
C6 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
ICC/IF image of ab74638 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74638, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab74638 は論文での使用が確認できていません。