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Full length native protein (purified) corresponding to Human C5b-9. Purified human SC5b-9 complex.
Our Abpromise guarantee covers the use of ab55811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|IHC-FoFr||Use at an assay dependent concentration.|
|RID||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab55811 staining C5b-9 (red) in Mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in 5% serum) for 2 hours. An Alexa Fluor® 546-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Insulin - green, DAPI - blue.
ab55811 at a 1/1000 dilution staining C5b-9 in mouse heart tissue by Immunohistochemistry (frozen sections), incubated for 16 hours at 4°C. Acetone fixed. Permeabilized using 1% Triton X-100. Blocked with 10% horse serum + 5% BSA for 1 hour at room temperature. Secondary used at 1/500 dilution polyclonal goat anti-rabbit IgG conjugated to Alexa Fluor® 488.
ICC/IF image of ab55811 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55811, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.