製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to C5b-9
  • 特異性
    This antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma
  • アプリケーション
    適用あり: IHC-Fr, IHC-P, IHC-FoFr, RID, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length native protein (purified) corresponding to Human C5b-9. Purified human SC5b-9 complex.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
RID Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells.
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • C5 antibody
    • c5b 9 antibody
    • C6 antibody
    • C7 antibody
    • C8 antibody
    • C9 antibody
    • Complement component 5 antibody
    • Complement component 6 antibody
    • Complement component 7 antibody
    • Complement component 8 antibody
    • Complement component 9 antibody
    see all

画像

  • ab55811 staining C5b-9 in human liver.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab55811 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55811, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Berg CT  et al. Influence of type I IFN signaling on anti-MOG antibody-mediated demyelination. J Neuroinflammation 14:127 (2017). IHC-P ; Mouse . Read more (PubMed: 28646890) »
  • Granados-Durán P  et al. Microbial Neuraminidase Induces a Moderate and Transient Myelin Vacuolation Independent of Complement System Activation. Front Neurol 8:78 (2017). Read more (PubMed: 28326060) »

See all 30 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
80 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 23 2014

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The datasheet pdfs can be downloaded from Abcam website.

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I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you.

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Wehope the second vial will work as it is expected, and please do letus know how you are getting on with this product.

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Thank you for contacting Abcam.

We have not tested ab55811 on rat tissue that does mean to say that it will not work, just that we have not tried it.

We are basing reactivity towards both human and mouse tissue based on in house tes...

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Thank you for your enquiry. The reason that the molecular weight is different could be that the C5-b-9 complex degrades under proteolysis and therefore on a western blot, we are only seeing degradation products. In this case, it is the phosphoryla...

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Thank you for your inquiry. I have contacted the originator of the ab55811 product and they have confirmed that this product was used in the following publication: Schafer, H., et al. 1986 (J. Immunol. 137, 1945), which suggests that this antibo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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