The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 mg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 188 kDa).
関連性C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kDa glycoprotein consisting of a disulphide linked alpha-chain (115kDa) and beta-chain (75kDa).
C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).
Anti-C5 / C5b antibody (ab46171) at 1 µg/ml + Human Complement C5 Protein at 0.1 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 188 kDa Observed band size : 74 kDa (why is the actual band size different from the predicted?) Complement component C5 protein is comprised of an alpha and beta chain which have predicted molecular weights of 115 kDa and 75 kDa respectively. ab46171 specifically detects the C5 beta chain.
Anti-C5 / C5b antibody (ab46171) 使用論文
has not yet been referenced specifically in any publications.