製品の概要

  • 製品名Anti-C5 / C5a antibody [557]
    C5 / C5a 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [557] to C5 / C5a
  • 特異性This antibody reacts with an epitope on C5 and C5a. The antibody 557 is capable to inhibit the binding of C5a to the C5a receptor through a competitive mechanism, it does not block the cleavage of C5 into C5a and C5b.
  • アプリケーション適用あり: WB, Inhibition Assay, Functional Studiesmore details
  • 種交差性
    交差種: Human
  • 免疫原

    BALB/c mice were immunized with human C5

  • エピトープAb11876 recognizes an epitope located within the alpha-chain of C5 that corresponds to C5a.
  • ポジティブ・コントロール
    • Recombinant C5

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.
Inhibition Assay Use at an assay dependent dilution.
Functional Studies Use at an assay dependent dilution.

ターゲット情報

  • 機能Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • 関連疾患Complement component 5 deficiency
    An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • 配列類似性Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Anaphylatoxin C5a analog antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 antibody
    • C5 antibody
    • C5a antibody
    • CO5_HUMAN antibody
    • Complement C5 alpha'' chain antibody
    • Complement C5 antibody
    • Complement component C5 antibody
    • CPAMD4 antibody
    see all

Anti-C5 / C5a antibody [557] 画像

  • ELISA with ab11876 as capture antibody at 1/100, ab11878 as detection antibody at 1/250, diluted in carbonate buffer or PBS. Dilutions of commercial C5a were used as the standard.

    See Abreview

Anti-C5 / C5a antibody [557] (ab11876) 使用論文

This product has been referenced in:
  • Wangdi T  et al. The Vi capsular polysaccharide enables Salmonella enterica serovar typhi to evade microbe-guided neutrophil chemotaxis. PLoS Pathog 10:e1004306 (2014). Mouse, Human . Read more (PubMed: 25101794) »
  • Kola A  et al. Epitope mapping of a C5a neutralizing mAb using a combined approach of phage display, synthetic peptides and site-directed mutagenesis. Immunotechnology 2:115-26 (1996). Read more (PubMed: 9373320) »

See all 2 Publications for this product

Product Wall

Thank you very much for your patience.

I have been checking with my lab colleagues the percentage of homology of the epitopes recognised by our anti C5 antibodies and the correspondent protein in bovine species. Unfortunately, I am afraid non...

Read More

Thank you for contacting us.

I have looked into this with the supplying lab:

Unfortunately we have no reference towards the statement. This has been communicated by a collaborator of the supplying lab upon the collaborator's intern...

Read More

Thank you for your enquiry. Unfortunately we could not find this article in our file. Is it possible to send us this article? Nevertheless we can confirm the clone mentioned in this article is the same as our product. The ab11876 recognizes an e...

Read More
Application ELISA
Sample Human Serum (Serum or EDTA plasma)
Specification Serum or EDTA plasma
Type Sandwich (Capture)
Username

Abcam user community

Verified customer

投稿 Nov 02 2007

Thank you for your enquiry. Ab11876 recognizes an epitope located within the alpha-chain of C5 that corresponds to C5a. Thus it recognizes both C5 and C5a. The antibody inhibits binding of C5a to the C5a receptor through a competitive mechanism. It...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"