製品の概要

  • 製品名Anti-C4d antibody
    C4d 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C4d
  • 特異性This antibody is unique in that it has been shown to detect both the C4 protein at 200kDa as well as the 40kDa C4d protein.
  • アプリケーション適用あり: ICC, Flow Cyt, ELISA, WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    KLH conjugated syn-thetic mouse C4 peptide fragment.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC
Flow Cyt
ELISA
WB
  • 追加情報ELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Detects a band of approximately 200,41 kDa (predicted molecular weight: 193 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
      Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • 関連疾患Defects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
    • 配列類似性Contains 1 anaphylatoxin-like domain.
      Contains 1 NTR domain.
    • 翻訳後修飾Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
      N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • acidic C4 antibody
      • Acidic complement C4 antibody
      • basic C4 antibody
      • basic complement C4 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
      • C4 antibody
      • C4, chido form antibody
      • C4, Rodgers form antibody
      • C4-1 antibody
      • C4a anaphylatoxin antibody
      • C4A antibody
      • C4A2 antibody
      • C4A3 antibody
      • C4A4 antibody
      • C4A6 antibody
      • C4AD antibody
      • C4b antibody
      • C4B_2 antibody
      • C4B1 antibody
      • C4B12 antibody
      • C4B2 antibody
      • C4B3 antibody
      • C4B5 antibody
      • C4BD antibody
      • C4d antibody
      • C4d fragment antibody
      • C4F antibody
      • C4S antibody
      • CH antibody
      • Chido form of C4 antibody
      • CO4 antibody
      • CO4A_HUMAN antibody
      • Complement C4 gamma chain antibody
      • complement C4-A antibody
      • complement C4-B antibody
      • complement C4-B-like antibody
      • complement C4B1a antibody
      • Complement component 4A antibody
      • complement component 4A (Rodgers blood group) antibody
      • Complement component 4B antibody
      • complement component 4B (Chido blood group) antibody
      • complement component 4B (Chido blood group), copy 2 antibody
      • complement component 4B (Chido blood group)-like antibody
      • Complement component 4F antibody
      • Complement component 4S antibody
      • CPAMD2 antibody
      • CPAMD3 antibody
      • DADB-112B14.11 antibody
      • MGC164979 antibody
      • RG antibody
      • Rodgers form of C4 antibody
      see all

    Anti-C4d antibody 画像



    • Predicted band size : 193 kDa
      Observed band size : 41,200 kDa (why is the actual band size different from the predicted?)

      Western blot analysis using ab89537. Serum from a C57BL6 mouse was aged for 0, 1 and 4 days at 37degrees C to allow cleavage of C4 to C4d. Blots reacted with ab89537 yielded both a 200 kDa band as well as a 41 kDa band corresponding to the molecular weight of C4d.

    Anti-C4d antibody (ab89537) 使用論文

    ab89537 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. I have been doing a little research into C4 and the antibodies that we currently have available against this target. As discussed on the phone, ab89537 recognises mouse C4d and C4 and has been tested in flow cytomet...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"