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Our Abpromise guarantee covers the use of ab36075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/80. Antigen retrieval with a citrate buffer system (or equivalent) is recommended.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/20 - 1/80.|
|IHC-Fr||1/20 - 1/80.|
Ab36075 staining C4d in Human kidney tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin embedded sections). Tissue was fixed with formaldehyde and heat mediated antigen retrieval was performed at 100°C with citrate for 20 minutes. Primary antibody was incubated at 1/80 dilution for 20 minutes at 25°C. A HRP conjugated anti-mouse IgG was used as a secondary antibody.
Ab36075 staining C4d in Human nasal sinus tissue by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 20% milk for 20 minutes. Primary antibody was incubated at a 1/80 dilution in PBS for 1 hour at 25°C. A biotin conjugated goat anti-rabbit was used as a secondary antibody at 1/100 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"