製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to C4b
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified Human C4b protein.

  • ポジティブ・コントロール
    • Human Placenta tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgY
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 193 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 0.1µg for 106 cells. ab37382-Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • 配列類似性
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 翻訳後修飾
    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basic complement C4 antibody
    • C2B5 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 antibody
    • C4b antibody
    • C4B1 antibody
    • C4B2 antibody
    • C4B3 antibody
    • CO4B_HUMAN antibody
    • Complement C4 gamma chain antibody
    • Complement component 4B antibody
    • CPAMD3 antibody
    see all

画像

  • ab118887, at 20 µg/ml, staining C4b in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

参考文献

ab118887 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab118887.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録