製品の概要

  • 製品名Anti-C4a antibody
    C4a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C4a
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Human C4a.

    (Peptide available as ab87430.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the Human plasma total protein

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 193 kDa).

Complement C4-A circulates in blood as a disulphide linked trimer of an alpha, beta and gamma chain (95, 75 and 33 kDa respectively). Our immunogen sequence is within the region corresponding to the alpha chain (95 kDa).

ターゲット情報

  • 機能C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • 関連疾患Defects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
  • 配列類似性Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 翻訳後修飾Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acidic complement C4 antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 2 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
    • C4a anaphylatoxin antibody
    • C4A antibody
    • C4AD antibody
    • CO4A_HUMAN antibody
    • Complement C4 A antibody
    • Complement C4 gamma chain antibody
    • Complement component 4A antibody
    • Complement component 4A preproprotein antibody
    • CPAMD2 antibody
    • RG antibody
    • Rodgers form of C4 antibody
    see all

Anti-C4a antibody 画像

  • Anti-C4a antibody (ab87429) at 2 µg/ml + Human Plasma Total Protein Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 193 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    Complement C4-A circulates in blood as a disulphide linked trimer of an alpha, beta and gamma chain (95, 75 and 33 kDa respectively). Our immunogen sequence is within the region corresponding to the alpha chain (95 kDa).

Anti-C4a antibody (ab87429) 使用論文

This product has been referenced in:
  • Hyung SW  et al. A serum protein profile predictive of the resistance to neoadjuvant chemotherapy in advanced breast cancers. Mol Cell Proteomics 10:M111.011023 (2011). WB ; Human . Read more (PubMed: 21799047) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"