製品の概要

  • 製品名Anti-C4a antibody
    C4a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to C4a
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Human C4a.

    (Peptide available as ab86260.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HepG2 Whole Cell Lysate and plasma total protein lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 193 kDa (predicted molecular weight: 193 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23236492

ターゲット情報

  • 機能C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • 関連疾患Defects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.
  • 配列類似性Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • 翻訳後修飾Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Acidic complement C4 antibody
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 2 antibody
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody
    • C4a anaphylatoxin antibody
    • C4A antibody
    • C4AD antibody
    • CO4A_HUMAN antibody
    • Complement C4 A antibody
    • Complement C4 gamma chain antibody
    • Complement component 4A antibody
    • Complement component 4A preproprotein antibody
    • CPAMD2 antibody
    • RG antibody
    • Rodgers form of C4 antibody
    see all

Anti-C4a antibody 画像

  • Anti-C4a antibody (ab66790) at 1 µg/ml + HepG2 (Human) Whole Cell Lysate (ab52257) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 193 kDa
    Observed band size : 193 kDa
  • Anti-C4a antibody (ab66790) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 193 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,41 kDa. We are unsure as to the identity of these extra bands.

Anti-C4a antibody (ab66790) 使用論文

This product has been referenced in:
  • Treutlein G  et al. Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis. PLoS One 7:e51391 (2012). WB, ICC/IF ; Cat . Read more (PubMed: 23236492) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - other (Human serum)
Total protein in input 500 µg
Immuno-precipitation step Other - Protein A and G Dynabeads and Protein A Agarose
Specification Human serum
Username

Dr. Shea Hamilton

Verified customer

投稿 Sep 30 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (brain homogenate)
Loading amount 75 µg
Specification brain homogenate
Gel Running Conditions Non-reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Aug 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"