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Our Abpromise guarantee covers the use of ab17453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/30000. Strong reaction is seen in ELISA with a C3 coat or when used as detection antibody in sandwich ELISA in combination with a polyclonal C3 antibody. ab17453 also reacts with C3b deposited on coated antibody molecules.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 187 kDa. In Western blotting after SDS-PAGE, ab17453 reacts with C3 in both reduced and unreduced forms.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Immunohistochemical analysis of human kidney labelling C3d with ab17453 at a dilution of 1/10. Plasma of veins were stained strongly.