アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
C3c complement isolated from complement activated human serum.
Fluorescein isothiocyanate (FITC) isomer 1.
Our Abpromise guarantee covers the use of ab4212 in the following tested applications.
|ICC/IF||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.
ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF image of ab4212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4212, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.